Recombinant SDHB Antibody - Primärer Antikörper, specific to SDHB, Rabbit IgG

Cat. No.: Ab126854

GRADE & PURITY Recombinant, ExactAb™, Validated, See COA

Application

IF/ICC, IP, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human SDHB Antibody, Recombinant, could be used for WB, IP and so on.

Specifications

Product Name

Recombinant SDHB Antibody - Primärer Antikörper, specific to SDHB, Rabbit IgG

Synonyme

CWS2 antibody | DHSB_HUMAN antibody | FLJ92337 antibody | Ip antibody | Iron sulfur subunit antibody | Iron sulfur subunit of complex II antibody | Iron-sulfur subunit of complex II antibody | mitochondrial antibody | PGL 4 antibody | PGL4 antibody | SDH

Spezifikationen & Reinheit

Recombinant, ExactAb™, Validated, See COA

Biochemische und physiologische Mechanismen

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

Wirtsspezies

Kaninchen

Spezifität

SDHB

Immunogen

A synthetic peptide derived from human SDHB (AA 1-280).

Positive Kontrolle

WB: Mouse slpeen, Rat spleen, HeLa, Jurkat and C6 lysates. IP: HeLa lysates. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant SDHB Antibody (Ab126854)

Recombinant SDHB Antibody (Ab126854) - Western Blot
All lanes: Recombinant SDHB Antibody (Ab126854) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 28, 24 kDa
The band observed at 24 kDa could potentially be a cleaved form of SDHB due to the presence of a 28 amino acid transit peptide.
Exposure time: 12.4 s

IP: Recombinant SDHB Antibody (Ab126854)

Recombinant SDHB Antibody (Ab126854) - IP
Immunoprecipitate (IP) analysis using Recombinant SDHB Antibody (Ab126854) at 1/50 dilution. (WB with Recombinant SDHB Antibody (Ab126854) at 1/1000 dilution).

IF/ICC: Recombinant SDHB Antibody (Ab126854)

Recombinant SDHB Antibody (Ab126854) - IF/ICC
IF analysis of Recombinant SDHB Antibody (Ab126854) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SDHB Antibody (Ab126854) at 1/300 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Anwendung

Anwendung

Dilution info

WB

1/1000 - 1/2000

IP

1/20-1/50

IF/ICC

1/300

Zugehörige Ziele (menschlich)

SDHB Tchem Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (0 Activities)

Discover Target: SDHB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant SDHB Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.