GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.
ExactAb™, Validated, Recombinant ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human VPS4a Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
Specifications
Product Name
Recombinant VPS4a Antibody - Primary antibody, specific to VPS4A, Rabbit IgG
Synonyms
Protein SKD2 antibody | hVPS4 antibody | SKD1 antibody | SKD1 homolog antibody | SKD1A antibody | SKD2 antibody | Vacuolar protein sorting 4 homolog A antibody | Vacuolar protein sorting 4, yeast, homolog of, A antibody | Vacuolar protein sorting factor 4
Specifications & Purity
ExactAb™, Validated, Recombinant
Host species
Rabbit
Specificity
VPS4A
Immunogen
A synthetic peptide derived from human VPS4a (AA 424-437).
Positive Control
WB: K562, HeLa, Jurkat, Hep G2, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant VPS4a Antibody (Ab134482) - Western Blot All lanes: Recombinant VPS4a Antibody (Ab134482) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 52 kDa Exposure time: 29.1 s
Recombinant VPS4a Antibody (Ab134482) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant VPS4a Antibody (Ab134482). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant VPS4a Antibody (Ab134482) - IF/ICC Immunocytochemistry analysis of VPS4a(green) in HeLa using Recombinant VPS4a Antibody (Ab134482), and DAPI(blue).
Application
Application
Dilution info
IF/ICC
1/50-1/200
WB
1/500-1/1000
IHC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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