BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Adenosine triphosphate (ATP) is the most fundamental carrier and the most direct source of energy in biological energy conversion. The determination of ATP content and the calculation of the energy charge can effectively reflect the energy metabolism status of an organism. This kit employs a creatine kinase‑coupled phosphomolybdic acid colorimetric method for the determination of ATP content. The principle is as follows: Under the catalysis of creatine kinase (CK), ATP reacts with creatine to generate phosphocreatine and ADP. Phosphocreatine is further hydrolyzed to inorganic phosphate (Pi). Under acidic conditions, Pi reacts with ammonium molybdate to form a phosphomolybdic acid complex, which is then reduced by a reducing agent (such as ferrous sulfate or ascorbic acid) to generate a blue molybdenum blue product. This product exhibits a characteristic absorption peak at 700 nm. By monitoring the increase in absorbance at 700 nm under substrate‑saturating conditions, the initial linear increase in absorbance per unit time (ΔOD₇₀₀/min) is directly proportional to the ATP content, allowing for accurate calculation of the ATP concentration in the sample.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View BioReagent grade guide →
