Src (phospho Y529) Antibody - Primary antibody, specific to Src (phospho Y529), Rabbit IgG

Cat. No.: Ab129131

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Rabbit

Reactive species

Human, Monkey, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit polyclonal to Src (phospho Y529), Polyclonal, could be used for WB, IHC and so on.  

Specifications

Product Name

Src (phospho Y529) Antibody - Primary antibody, specific to Src (phospho Y529), Rabbit IgG

Synonyms

Src (phospho Y529) | p-Src (phospho Y529) | Src(Phospho-Tyr529) | ASV | Avian sarcoma virus | c SRC | CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA | cSrc | EC 2.7.10.2 | Neuronal CSRC tyr

Specifications & Purity

Validated, ExactAb™, 1.0 mg/mL

Biochemical and Physiological Mechanisms

Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-

Host species

Rabbit

Specificity

Src (phospho Y529)

Immunogen

A synthetic peptide derived from human Src (phospho Y529) (AA 487-536).

Positive Control

WB: A549, NIH/3T3 lysates. IHC: Human breast carcinoma tissue.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Src (phospho Y529) Antibody (Ab129131)

Src (phospho Y529) Antibody (Ab129131) - Western Blot
All lanes: Src (phospho Y529) Antibody (Ab129131) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
Exposure time: 3.0 s

Western Blot: Src (phospho Y529) Antibody (Ab129131)

Src (phospho Y529) Antibody (Ab129131) - Western Blot
All lanes: Src (phospho Y529) Antibody (Ab129131) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
Exposure time: 5.0 s

IHC: Src (phospho Y529) Antibody (Ab129131)

Src (phospho Y529) Antibody (Ab129131) - IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Src (phospho Y529) Antibody (Ab129131). The picture on the right is blocked with the phospho peptide.

ELISA: Src (phospho Y529) Antibody (Ab129131)

Src (phospho Y529) Antibody (Ab129131) - ELISA
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Src (phospho Y529) Antibody (Ab129131).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/100-1/300

Associated Targets(Human)

SRC Tclin Proto-oncogene tyrosine-protein kinase Src (0 Activities)

Discover Target: SRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

SRC Tclin Tyrosine-protein kinase SRC (10310 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SRC Tclin VEGF-receptor 2 and tyrosine-protein kinase SRC (25 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SRC Tclin Calmodulin/Proto-oncogene tyrosine-protein kinase Src (2 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SRC Tclin VHL/SRC (1 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SRC Tclin Src/Lck kinase (1 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action