SDS-PAGE

Electrophoresis buffers determine sample migration speed, band resolution, heat generation, and subsequent recovery efficiency. When selecting a buffer, it is necessary to first distinguish whether the sample is DNA, RNA, or protein, and then determine the buffer system according to the target ...

Standard Operating Procedure (SOP) for Bis-Tris Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Common Questions and Answers for SDS-PAGE Experiments

The primary function of β-mercaptoethanol is to selectively cleave disulfide bonds in protein molecules—chemical bonds that are critical for maintaining the protein’s spatial conformation. Once disrupted, these bonds cause partial dissociation of the protein’s secondary and higher-order ...

Polyacrylamide gel electrophoresis (PAGE) is an electrophoretic technique that employs polyacrylamide gel as the supporting matrix. The core principle lies in the molecular sieving effect of the gel’s network structure, which enables precise separation of proteins and nucleic acids based on ...

Protein blotting (WB) is widely used to analyze the expression of specific proteins in cell or tissue extracts. Western blot experiments are time-consuming, and their success has a significant impact on research progress.

Protein dephosphorylation is an important biochemical process in cells, involving the removal of phosphate groups from specific amino acid residues on proteins. This process corresponds to protein phosphorylation, and the two together form a dynamic balance that is crucial for cell signal ...

The Interleukin (IL) family is a group of cytokines and development factors mainly derived from white blood cells, which play a key role in coordinating the immune response.