Sucrose synthase (OcSUS1), CAS No.9030-05-1

CAS: 9030-05-1 Cat. No.: S1525149

GRADE & PURITY Bioactive, Recombinant, EnzymoPure™, expressed in E.coli; ≥0.5 U/mg protein; Protein concentration: See COA

Accession #

P31922

Expression system

E. coli

Bioactivity

≥0.5 U/mg protein

Overview

Sucrose synthase (OcSUS1), E.C 2.4.1.13, derived from Ornithogalum caudatum. This enzyme is applied for the synthesis of UDP-glucose. The product is recombinantly expressed in Escherichia coli with high purity and suitable for one-pot multi-enzyme systems.

Reaction Equation

Neu5Ac + CTP —— CMP-Neu5Ac + PPi

Usage Instructions

1.Add substrates and 5 mM MgCl₂ to the reaction system. Supplement buffer salts (e.g., Tris-HCl) as needed to adjust pH to 7, then add an appropriate amount of OcSUS1 according to reaction requirements.

2.Mix thoroughly and incubate the sample at 37 °C for 1–2 h. Tris-HCl (pH 7) is the recommended reaction buffer; most common biological buffers such as Tris and PBS are also compatible.

3.Terminate the reaction by heat inactivation in a boiling water bath for 5 min, centrifuge at 12,000 rpm for 10 min, and collect the supernatant for detection.

Notes

For scale-up reactions, incubation duration or enzyme dosage shall be adjusted based on actual conditions; gentle shaking is recommended for uniform mixing.

Most conventional biological buffers including Tris and PBS are applicable. Buffers outside this pH range (e.g., acetate buffer) may also work, yet incubation time and enzyme loading need to be optimized experimentally.

Specifications

Product Name

Sucrose synthase (OcSUS1), CAS No.9030-05-1

Grade

Bioactive, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, EnzymoPure™, expressed in E.coli; ≥0.5 U/mg protein; Protein concentration: See COA

Bioactivity

≥0.5 U/mg protein

Accession #

P31922

Predicted molecular weight

86 kDa

CAS

9030-05-1

Enzyme Commission Number

EC 2.4.1.13

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in E.coli; ≥0.5 U/mg protein; Protein concentration: See COA

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.