Sumo 1 Antibody - Primärer Antikörper, specific to Sumo 1, Rabbit IgG

Cat. No.: Ab129741

GRADE & PURITY ExactAb™, Validated, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human Sumo 1 Antibody, Polyclonal, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/1000

IHC: 1/50-1/100

Protein Function:

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.

Specifications

Product Name

Sumo 1 Antibody - Primärer Antikörper, specific to Sumo 1, Rabbit IgG

Synonyme

DAP1 antibody | GAP modifying protein 1 antibody | GAP-modifying protein 1 antibody | GMP 1 antibody | GMP1 antibody | OFC10 antibody | PIC 1 antibody | PIC1 antibody | SENP2 antibody | Sentrin 1 antibody | Sentrin antibody | Small ubiquitin related modif

Spezifikationen & Reinheit

ExactAb™, Validated, 0.6 mg/mL

Wirtsspezies

Kaninchen

Spezifität

Sumo 1

Immunogen

A synthetic peptide derived from human Sumo 1 (AA 84-96)

Positive Kontrolle

WB: HeLa, MCF7, A549, NIH/3T3 and C6 cell lysates. IHC: Human breast carcinoma tissue. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

0.6 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Sumo 1 Antibody (Ab129741)

Sumo 1 Antibody (Ab129741) - Western Blot
All lanes: Sumo 1 Antibody (Ab129741) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 12 kDa
Observed band size: 18, 82 kDa
Exposure time: 56.1 s

IHC: Sumo 1 Antibody (Ab129741)

Sumo 1 Antibody (Ab129741) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Sumo 1 Antibody (Ab129741). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Sumo 1 Antibody (Ab129741)

Sumo 1 Antibody (Ab129741) - IF/ICC

IF analysis of Sumo 1 Antibody (Ab129741) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Sumo 1 Antibody (Ab129741) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Anwendung

Anwendung

Dilution info

WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/200

Zugehörige Ziele (menschlich)

SUMO1 Tbio Small ubiquitin-related modifier 1 (0 Activities)

Discover Target: SUMO1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Sumo 1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.