Synthetic Blocking Buffer, ELISA - Ready-to-use (RTU)

Cat. No.: S494401

GRADE & PURITY Ready-to-use (RTU)

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Übersicht

Product Characteristics 

The Synthetic Blocking Buffer, ELISA is a ready-to-use solution for blocking of the remaining free binding sites after microplate coating in ELISAs. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background signal, improving the signal-to-noise ratio. 

The Synthetic Blocking Buffer, ELISA reduces the risk of false positive reactions in the assay, while avoiding the current safety hazards related to the use of bovine biological material, for instance BSA.

Composition & Properties 

The Synthetic Blocking Buffer is a ready-to-use buffer. The buffer contains no proteins, and crossreactivity is therefore avoided.

Working Procedure 

1.Coat the microtiter plate according to the usual procedure. It is recommended to dilute the biomolecule in a fresh carbonate buffer, pH 9.6. 

2.After plate coating, rinse the microwells with 3 x 300 µl PBS buffer. 

3.Dispense the Synthetic Blocking Buffer into each well making sure wells are completely full. 

4.Incubate for 5-10 minutes at room temperature. 

5.Rinse with 3 x 300 µl washing buffer optimized for the assay. 

6.Continue the plate processing using the usual procedure.

Tips & Tricks 

• In case of a demand for stabilization of coated ELISA plates, our one step blocker & stabilizer, WellChampion (cat. no. W494467) is recommended. 

• For membranes, the Synthetic Blocking Buffer, Blotting (cat. no. S494457) is recommended. 

Handling & Storage 

• Store solution at 2-8 °C.

Specifications

Spezifikationen & Reinheit

Ready-to-use (RTU)

Storage

Store at 2-8°C,Protected from light

Verschickt in

Wet ice

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Chemische und physikalische Eigenschaften

• Empfindlichkeit: Light sensitive

Häufig gestellte Fragen

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.