Thrombin, CAS No.9002-04-4

CAS: 9002-04-4 Cat. No.: T754968 Molecular Weight: 263.75 EC Number: 232-648-7 PubChem CID: 65045

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from human plasma; 400-1000 NIH U/mg protein

Accession #

P00734

Bioactivity

400-1000 NIH U/mg protein

Overview

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Lyophilized powder containing sucrose, sodium chloride and Tris.

Application:

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an innovative flow cytometric approach for small-size platelet microparticles.

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard.

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Specifications

Product Name

Thrombin, CAS No.9002-04-4

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from human plasma; 400-1000 NIH U/mg protein

Biochemical and Physiological Mechanisms

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation

Bioactivity

400-1000 NIH U/mg protein

Accession #

P00734

CAS

9002-04-4

Enzyme Commission Number

3.4.21.5

Molecule Type

Enzyme

Product Properties

• Nacres: NA.54

Storage and Shipping

Concentration

from human plasma; 400-1000 NIH U/mg protein

Reconstitution

Reconstitute in 1% buffered Albumin solution.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard. The NIH assay procedure uses 0.2 mL plasma diluted 1:1 with saline as substrate and 0.1 mL of Thrombin sample diluted in 1% buffered Albumin solution. B

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