Polyacrylamide gel electrophoresis of serum proteins
Serum protein polyacrylamide gel has the advantages of good mechanical strength, elasticity, transparency, high chemical stability, non-electroosmosis, simple e
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Urea gradient polyacrylamide gel electrophoresis and folding analysis experiments
Goldenberg and Creighfn (1984) introduced the use of polyacrylamide gels containing a concentration gradient of urea to analyze the folded state of proteins. A
DEAE SEPHADEX A-50 anion-exchange chromatography assay for calmodulin
The next step in the purification of calmodulin is anion exchange chromatography with DEAE resin. This step takes advantage of the fact that calmodulin is a str
Experimental extraction of calmodulin from chicken gizzard
Calmodulin is found in all mammalian tissues and also in other eukaryotic organisms. It is one of the most ubiquitous and highly conserved proteins known. This
Protein sequence analysis and structure prediction experiments
Source of content: Guangdong Pharmaceutical University Laboratory Instruction Manual.
Titration curve experiment
The titration curve is a curve presenting the mobility of each component of the sample against pH, for which the gel containing the carrier amphoteric electroly
Crude graded separation experiments of calmodulin
For the purification of calmodulin, two different operations were performed for the crude graded separation of smooth muscle tissue extracts: ammonium sulfate p
Protein ultraviolet spectrometry assay
Since the benzene rings of tyrosine and tryptophan residues in protein molecules contain conjugated double bonds, proteins have the property of absorbing ultrav
Phenyl-Sepharose chromatographic assay of calmodulin
This protocol has been developed over many years of practice, and it is not an example of a first attempt to purify a protein by HIC chromatography. For calmodu