Gel mobility change analysis experiment
In this experiment, we used the same complementary oligonucleotide used for DNA affinity chromatography (see the end of the Introduction to Experiment 3 for the
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Experiments on purification of oligonucleotides by preparative gel electrophoresis
This experiment describes how to purify oligonucleotides by preparative gel electrophoresis. This experiment is from Protein Purification and Identification Lab
Quantitative protein spot blotting assay
Protein blotting, which uses immunological methods to measure the amount of a particular protein. This experiment was derived from Protein Purification and Iden
Sephacryl S-300 HR column chromatography of HeLa cell nucleus extracts
This experiment describes the process of Sephacryl S-300 HR column chromatography of HeLa cell nucleus extract. This experiment is from the guide to protein pur
immunoaffinity chromatography
This experiment introduces the method of immunoaffinity chromatography. This experiment is from the Laboratory Guide for Protein Purification and Identification
Heparin-Sepharose CL-2B preparation experiment
This experiment describes the preparation of heparin-Sepharose CL-2B. This experiment was derived from the Experimental Guide for Protein Purification and Ident
Precipitation σ32 and RNA polymerase assays
This experiment describes the amount of polyethyleneimine needed to precipitate σ32 and RNA polymerase. This experiment was derived from Protein Purification a
Screening experiments of descaling agents for solubilizing membrane proteins
Although TriumX-100 is a good descaling agent for solubilizing insulin receptors from murine liver plasma membranes, it may not be suitable for solubilization o
Insulin receptor solubilization and activity assay experiments
This experiment describes about the process of solubilization and activity determination of insulin receptor. This experiment is from the guide to protein purif