3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
The purpose of this section is to provide an abbreviated set of working procedures for how to use a series of experiments similar to those described in this uni
This experiment describes the process of lectin affinity chromatography on solubilized receptors. This experiment was derived from Protein Purification and Iden
Oxidation of proteins with peroxyformic acid is a simple and effective method of partially denaturing proteins and stabilizing sulfur-containing amino acids. Pe
In this experiment, we will examine the insulin-stimulated tyrosine kinase activity of the insulin receptor. The phosphorylated receptor will be detected with a
Another useful alternative to TCA precipitation is the use of an acidified acetone-methanol mixture. Acidification helps to remove SDS from the sample by dissol
One of the most important aspects of molecular biology is the analysis of trace proteins, making possible the cloning of cDNA sequences encoding the proteins to
This experiment describes about the analysis of insulin receptor glycosylation. This experiment is from the Protein Purification and Identification Lab Guide by
Here, we introduce this procedure by taking the coupling of ligand to Affi-Gel10 as an example. This experiment was derived from Protein Purification and Identi
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