3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
When a protein is electrophoresed under conditions that keep its natural conformation constant, its mobility can be used as a measure of its homogeneity. Since
Insulin binds to its receptor with high affinity (KD~109 mol/L), and this ability allows us to estimate the number of insulin receptors in even fairly crude pre
This experiment describes the Neville method for isolation of liver plasma membrane. This experiment is from the guide to protein purification and characterizat
Protein concentrations have traditionally been determined by the method of Lowry and co-workers (Lowry et al., 1951) using the Folin-Ciocalteau reagent, but thi
The method described by Neville (1968) works well for isolating murine liver plasma membranes, but is not necessarily applicable for isolating plasma membranes
In routine protein spot blotting, the blotting membrane is closed for 1 hour, reacted with primary antibody for 1 hour, and then reacted with secondary antibody
This experiment describes how much time it takes for a protein to bind to an immunoaffinity medium. This experiment is from the Protein Purification and Identif
This experiment describes the amount of sodium N-dodecyl sarcosinate required for solubilizing inclusion body precipitation. This experiment is from the Laborat
Since proteins can be separated with high resolution on SDS gels and sensitive staining can be given with Caumas Brilliant Blue (CBB), the purity of the protein
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