silver dyeing experiments
This experiment describes the process of gel staining silver staining. This experiment is from the Protein Purification and Identification Laboratory Guide by Z
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Separation of phosphopeptides with iminodiacetic acid (iron chelate) Sepharose 6B
In the structural and functional analysis of proteins (phosphorylated proteins), it is useful to be able to separate phosphopeptides from unmodified (unphosphor
Precipitation experiment with trichloroacetic acid
TCA precipitation experiments are mainly used for (1) precipitation purification of proteins and (2) extraction of specimen compounds.
Cross-linking experiments of the insulin receptor with [125I]insulin
This experiment describes about the cross-linking process of insulin receptor with [125I] insulin. This experiment is from the Experimental Guide for Protein Pu
Insulin affinity chromatography assay
In this experiment, we used insulin agarose column chromatography as the final step in insulin receptor purification. This method utilizes the high affinity bin
Simplified oligonucleotide gene mixing assay
Improvements in the biochemical properties of proteases can be achieved through misdirected mutagenesis and DNA hybridization of the enzyme genes. The hybridiza
Determination of isoelectric point of proteins by isoelectric focusing electrophoresis
Isoelectric focusing (IEF for short) is a new technique that appeared in the mid-sixties. In recent years, IEF has made new progress and has rapidly developed i
Integral adulteration experiments of non-natural amino acids in Escherichia coli
The doping of amino acid analogs is becoming increasingly useful. The targeted incorporation of non-natural amino acids has made it possible to use chemical bio
Experiments on combinatorial protein design strategies using computational methods
Computational methods have always played an important role in protein design. This work focuses on searching the protein sequence space to find one or several p