3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
The use of DNA mashups to mimic natural evolutionary processes is a common approach to optimize DNA and protein properties. Here we present a new development in
Despite their apparent simplicity, coiled coil (Coiled Coil) modules are highly specialized and are important in understanding tertiary structure and its format
Among DNA-binding modulators, (Cys)2(His)2-type zinc-finger modulators have a large potential for manipulation. Zinc finger modulators provide an attractive fra
We developed the use of the 10th fibronectin type III structural domain (FNfnlO) of human fibronectin as a framework to show the use of multiple surface chain s
When designing protein libraries for screening, we must do everything possible to generate a diversity of protein variants within the physical limits of what ca
Calmodulin (CaM) is a ubiquitously distributed protein involved in calcium-involved signaling. Upon Ca2+ influx, CaM acquires a strong affinity for binding vari
Site-specific nucleic acid endonucleases are involved in many aspects of nucleic acid biochemistry. Restriction enzymes and related enzymes have become the para
Compartmentalized self-replication (CSR) is a novel approach to the directed evolution of proteases, especially polymerases. In the simplest form of CSR, it con
Improving enzyme stability is an ideal design step for modifying enzymes to exercise their activity under extreme conditions, such as high temperatures, as well
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