HEPES - Suitable for molecular biology, ≥99.5%(T) , CAS No.7365-45-9

CAS: 7365-45-9 Cat. No.: H109408 Formula: C₈H₁₈N₂O₄S Molecular Weight: 238.30 EC Number: 230-907-9

GRADE & PURITY Suitable for molecular biology, ≥99.5%(T)

Synonyms

HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), >=99.5% (titration) | 1d1p | STK033062 | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid | HEPES, biochemical grade | 4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid) | AMY23345 | DTXSID5040382 | Epit

Storage

Room temperature

Shipped In

Normal

Overview

HEPES has been described as one of the best all-purpose buffers available for biological research.At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Specifications

Synonyms

HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), >=99.5% (titration) | 1d1p | STK033062 | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid | HEPES, biochemical grade | 4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid) | AMY23345 | DTXSID5040382 | Epit

Specifications & Purity

Suitable for molecular biology, ≥99.5%(T)

Storage

Room temperature

Shipped In

Normal

Grade

Suitable for molecular biology

Purity

≥99.5%(T)

Names and Identifiers

Pubchem Sid

488182841

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/488182841

Canonical Smiles

C1CN(CCN1CCO)CCS(=O)(=O)O

IUPAC Name

2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

InChIKey

JKMHFZQWWAIEOD-UHFFFAOYSA-N

INCHI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

Isomeric SMILES

C1CN(CCN1CCO)CCS(=O)(=O)O

RTECS

TL6809000

Molecular Weight

238.30

Reaxy-Rn

883043

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=883043&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Diazinanes
      • Subclass Piperazines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Diazinanes
• Subclass: Piperazines
• Intermediate Tree Nodes: Not available
• Direct Parent: N-alkylpiperazines
• Alternative Parents: Sulfonyls Organosulfonic acids Alkanesulfonic acids Trialkylamines 1,2-aminoalcohols Azacyclic compounds Primary alcohols Organopnictogen compounds Organic zwitterions Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aliphatic heteromonocyclic compounds
• Substituents: N-alkylpiperazine - Organic sulfonic acid or derivatives - Organosulfonic acid or derivatives - Organosulfonic acid - Sulfonyl - Alkanesulfonic acid - 1,2-aminoalcohol - Tertiary amine - Tertiary aliphatic amine - Alkanolamine - Azacycle - Hydrocarbon derivative - Organic oxide - Organopnictogen compound - Organic oxygen compound - Primary alcohol - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Amine - Organic nitrogen compound - Alcohol - Organic zwitterion - Aliphatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as n-alkylpiperazines. These are organic compounds containing a piperazine ring where the nitrogen ring atom carries an alkyl group.
• External Descriptors: organosulfonic acid - HEPES

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Mechanisms of Action

Chemical and Physical Properties

• Melt Point(°C): 210 °C
• Molecular Weight: 238.310 g/mol
• XLogP3: -4.000
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 5
• Exact Mass: 238.099 Da
• Monoisotopic Mass: 238.099 Da
• Topological Polar Surface Area: 89.500 Ų
• Heavy Atom Count: 15
• Formal Charge: 0
• Complexity: 267.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1