HEPES solution(HEPES) - for cell culture, CellGuard Certified, 1 M, 0.2 μm filtered , CAS No.7365-45-9

CAS: 7365-45-9 Cat. No.: H755905 Formula: C₈H₁₈N₂O₄S Molecular Weight: 238.30 EC Number: 230-907-9

GRADE & PURITY for Cell culture, CellGuard Certified, 1 M, 0.2 μm filtered

Storage

Room temperature

Shipped In

Normal

Overview

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application:

RNAse HEPES has been used:

• To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
• To recover purified ribonucleotide
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

Specifications

Specifications & Purity

for cell culture, CellGuard Certified, 1 M, 0.2 μm filtered

Useful PH Range

6.8-8.2

Storage

Room temperature

Shipped In

Normal

Grade

CellGuard Certified, for Cell culture

Names and Identifiers

PH

5.0-6.0

Isomeric SMILES

C1CN(CCN1CCO)CCS(=O)(=O)O

RTECS

TL6809000

Molecular Weight

238.30

Reaxy-Rn

883043

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=883043&ln=

Chemical and Physical Properties

• Melt Point(°C): 210 °C