CD45 Mouse mAb - Primary antibody, specific to CD45, Mouse IgG1

Cat. No.: Ab094902

GRADE & PURITY Carrier Free, Validated, ExactAb™, See COA

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

HI30

Conjugation

Unconjugated

Overview

Mouse anti Human CD45 Antibody, Monoclonal (HI30), could be used for Flow and so on.
Application:
Flow: < 0.5 μg/100w cells
Protein Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Specifications

Product Name

CD45 Mouse mAb - Primary antibody, specific to CD45, Mouse IgG1

Synonyms

B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-protein phosphatase C | T

Specifications & Purity

Carrier Free, ExactAb™, Validated, See COA

Host species

Mouse

Specificity

CD45

Immunogen

Peripheral blood mononuclear cells

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Shipped at 4°C. Store at 4°C long term (36 months). Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Raji cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Jurkat cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 with CD45 Mouse mAb (Ab094902) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 (red) with CD45 Mouse mAb (Ab094902) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow:

  < 0.5µg/100w cells;

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action