Recombinant CD45RO Antibody - Anticuerpo primario, specific to Human CD45RO

Cat. No.: Ab212153

CALIDAD & PUREZA Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA

Application

ELISA, Flow Cytometry

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2a

Clone Number

P08F59

Conjugation

Unconjugated

Descripción general

Recombinant CD45RO Antibody, clone P08F59, could be used for Flow Cytometry, ELISA and so on.

Especificaciones

Product Name

Recombinant CD45RO Antibody - Anticuerpo primario, specific to Human CD45RO

Sinónimos

B220 | CD45 antigen | CD45R | CD45RO | EC 3.1.3.48 | L-CA | LY5 | protein tyrosine phosphatase, receptor type, C | receptor-type tyrosine-protein phosphatase C | T200 glycoprotein | T200 leukocyte common antigen | T200receptor type, c polypeptide | B220 a

Especificaciones y pureza

Animal Free,Carrier Free,Recombinant,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),See COA

Mecanismos bioquímicos y fisiológicos

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second

Especies hospedadoras

Ratón

Especificidad

Human CD45RO

Inmunógeno

IL-2 dependent T cell line, CA1

Control positivo

Flow Cytometry: Human peripheral blood lymphocytes

Conjugación

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Recombinant, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Flow Cytometry: Recombinant CD45RO Antibody (Ab212153)

Recombinant CD45RO Antibody (Ab212153) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45RO (red) with Recombinant CD45RO Antibody (Ab212153) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Recombinant CD45RO Antibody (Ab212153)

Recombinant CD45RO Antibody (Ab212153) - SEC
The purity of Recombinant CD45RO Antibody (Ab212153) is more than 95% verified by HPLC.

Aplicación

Aplicación

Dilution info

Flow Cytometry

≤0.5 µg per million cells in 100 µl volume

Objetivos asociados (humanos)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD45RO Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.