Recombinant CD45RO Antibody (AF555) - Anticuerpo primario, specific to Human CD45RO

Cat. No.: Ab213944

CALIDAD & PUREZA ExactAb™, Validated, 5 μL/test

Application

Flow Cytometry

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2a

Clone Number

P08F59

Conjugation

AF555

Descripción general

Recombinant CD45RO Antibody, clone P08F59, could be used for Flow Cytometry.

Especificaciones

Product Name

Recombinant CD45RO Antibody (AF555) - Anticuerpo primario, specific to Human CD45RO

Sinónimos

B220 | CD45 antigen | CD45R | CD45RO | EC 3.1.3.48 | L-CA | LY5 | protein tyrosine phosphatase, receptor type, C | receptor-type tyrosine-protein phosphatase C | T200 glycoprotein | T200 leukocyte common antigen | T200receptor type, c polypeptide | B220 a

Especificaciones y pureza

ExactAb™, Validated, 5 μL/test

Mecanismos bioquímicos y fisiológicos

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the secon

Especies hospedadoras

Ratón

Especificidad

Human CD45RO

Inmunógeno

IL-2 dependent T cell line, CA1

Control positivo

Flow Cytometry: Human peripheral blood lymphocytes.

Conjugación

AF555

Excitación (nm)

Green Laser(532nm)

Emisión (nm)

568nm

Ex/Em(nm)

Ex:553nm;Em:568nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

5 μL/test

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: Recombinant CD45RO Antibody (AF555) (Ab213944)

Recombinant CD45RO Antibody (AF555) (Ab213944) - Flow Cytometry
Flow cytometry analysis of CD45RO expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45RO Antibody (AF555) (Ab213944) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF555) (Ab190246).

Flow Cytometry: Recombinant CD45RO Antibody (AF555) (Ab213944)

Recombinant CD45RO Antibody (AF555) (Ab213944) - Flow Cytometry
Flow cytometry analysis of CD45RO expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45RO Antibody (AF555) (Ab213944) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Objetivos asociados (humanos)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD45RO Antibody (AF555)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.