CD45 Mouse mAb (Biotin) - Anticuerpo primario, specific to CD45, Mouse IgG1

Cat. No.: Ab179532

CALIDAD & PUREZA ExactAb™, Azide Free, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

HI30

Conjugation

Biotin

Descripción general

Application:

Flow: ≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Especificaciones

Product Name

CD45 Mouse mAb (Biotin) - Anticuerpo primario, specific to CD45, Mouse IgG1

Sinónimos

B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-protein phosphatase C | T

Especificaciones y pureza

ExactAb™, Validated, Azide Free, 0.5 mg/mL

Especies hospedadoras

Ratón

Especificidad

CD45

Inmunógeno

Peripheral blood mononuclear cells

Control positivo

Flow: Jurkat

Conjugación

Biotin

Grado

Azide Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD45 Mouse mAb (Biotin) (Ab179532)

CD45 Mouse mAb (Biotin) (Ab179532) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Objetivos asociados (humanos)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD45 Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.