Recombinant CD45RA Antibody (AF700) - Anticuerpo primario, specific to CD45RA

Cat. No.: Ab213933

GRADE & PURITY ExactAb™, Validated, 5 μL/test

Application

Flow Cytometry

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2b

Clone Number

P02I11

Conjugation

AF700

Descripción general

Recombinant CD45RA Antibody, clone P02I11, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD45RA Antibody (AF700) - Anticuerpo primario, specific to CD45RA

Sinónimos

CD34 antibody | CD34 antigen antibody | CD34 molecule antibody | CD34_HUMAN antibody | Cluster designation 34 antibody | cluster of differentiation 34 antibody | Hematopoietic progenitor cell antigen CD34 antibody | HPCA1 antibody | Mucosialin antibody |

Especificaciones y pureza

ExactAb™, Validated, 5 μL/test

Mecanismos bioquímicos y fisiológicos

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affec

Especies hospedadoras

Ratón

Especificidad

CD45RA

Inmunógeno

Low buoyancy density human lymphocytes

Control positivo

Flow Cytometry: Human peripheral blood lymphocytes

Conjugación

AF700

Excitación (nm)

Red Laser(633nm)

Emisión (nm)

719nm

Ex/Em(nm)

Ex:696nm;Em:719nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

5 μL/test

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: Recombinant CD45RA Antibody (AF700) (Ab213933)

Recombinant CD45RA Antibody (AF700) (Ab213933) - Flow Cytometry
Flow cytometry analysis of CD45RA expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45RA Antibody (AF700) (Ab213933) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF700) (Ab190248).

Recombinant CD45RA Antibody (AF700) (Ab213933) - Flow Cytometry Flow cytometry analysis of CD45RA expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45RA Antibo

Recombinant CD45RA Antibody (AF700) (Ab213933) - Flow Cytometry
Flow cytometry analysis of CD45RA expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45RA Antibody (AF700) (Ab213933) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF700) (Ab190248). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Objetivos asociados (humanos)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD45RA Antibody (AF700)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.