DTT Solution - 1M in H2O , CAS No.3483-12-3

CAS: 3483-12-3 Cat. No.: D301909 Fórmula: C₄H₁₀O₂S₂ Peso molecular: 154.25 Beilstein Registry Number: 1719760 Número EC: 222-468-7

GRADE & PURITY 1M in H2O

Synonyms

DTT | AKOS005259570 | Threitol, 1,4-dithio-, L- | (+/-)-1,4-Dimercapto-2,3-butanediol | 2-propylpenten-2-oic Acid | AKOS015855399 | MFCD00004877 | dithio threitol | AC-37037 | DL-Dithiothreitol, >=99.0% (RT) | 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel-

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

Product introduction

DTT is similar to mercaptoethanol, and it is a reducing agent and deprotection agent for sulfhydryl DNA. 1M DTT solution (in water) is often used as a basic component to reduce disulfide bonds in protein, which has antioxidant effect. The pungent odor and toxicity of DTT are much lower than those of mercaptoethanol.

Usage

1 according to the specific requirements of the experiment.

Scope of use

Applicable to general biology and medical research

Product use

Dithiothreitol is an effective reducing agent for protein analysis. When preparing samples for electrophoresis or other applications, sealed ampoules are more convenient and easy to use, and waste and loss of efficacy can be avoided.

Specifications

Sinónimos

DTT | AKOS005259570 | Threitol, 1,4-dithio-, L- | (+/-)-1,4-Dimercapto-2,3-butanediol | 2-propylpenten-2-oic Acid | AKOS015855399 | MFCD00004877 | dithio threitol | AC-37037 | DL-Dithiothreitol, >=99.0% (RT) | 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel-

Especificaciones y pureza

1M in H2O

Mecanismos bioquímicos y fisiológicos

Cell-permeable reducing reagent. Prevents oxidation of thiol groups. Protective and protein purification agent. Active in vivo and in vitro .

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Nota

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Nombres e identificadores

Pubchem Sid

528764245

Sonrisas canónicas

O[C@H](CS)[C@H](O)CS

IUPAC Name

(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol

InChIKey

VHJLVAABSRFDPM-QWWZWVQMSA-N

INCHI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

Isómeros SMILES

C([C@H]([C@@H](CS)O)O)S

WGK Alemania

3

RTECS

EK1610000

CAS alternativo

27565-41-9

Peso molecular

154.25

Beilstein

1719760

Reaxy-Rn

9483598

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=9483598&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic oxygen compounds
    • Clase Organooxygen compounds
      • Subclass Alcohols and polyols
        • Level5 Polyols
          • Level6 1,2-diols

• Kingdom: Organic compounds
• Superclass: Organic oxygen compounds
• Clase: Organooxygen compounds
• Subclass: Alcohols and polyols
• Intermediate Tree Nodes: Polyols
• Direct Parent: 1,2-diols
• Alternative Parents: Secondary alcohols Alkylthiols Hydrocarbon derivatives
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: Secondary alcohol - 1,2-diol - Alkylthiol - Hydrocarbon derivative - Organosulfur compound - Aliphatic acyclic compound
• Descripción: This compound belongs to the class of organic compounds known as 1,2-diols. These are polyols containing an alcohol group at two adjacent positions.
• External Descriptors: 1,4-dithiothreitol

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Objetivos asociados (no humanos)

Trypanosoma brucei brucei (13300 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

pgaB Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (23 Activities)

Discover Target: pgaB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción

Propiedades químicas y físicas

• Solubilidad: H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow
• Sensibilidad: Air & Heat and Moisture Sensitive
• Punto de inflamación (°F): 235.4 °F
• Punto de inflamación (°C): 113 °C
• Punto de ebullición (°C): 125～130°C
• Punto de fusión (°C): 42-43°C
• Peso molecular: 154.300 g/mol
• XLogP3: -0.400
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 3
• Exact Mass: 154.012 Da
• Monoisotopic Mass: 154.012 Da
• Topological Polar Surface Area: 42.500 Ų
• Heavy Atom Count: 8
• Formal Charge: 0
• Complexity: 52.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 2
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1