CD22 Mouse mAb - Anticorps primaire, specific to CD22, Mouse IgG1

Cat. No.: Ab094278

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Depletion, Flow Cytometry, Functional Assay, IP

Host species

Souris

Reactive species

Souris

Isotype

Mouse IgG1

Clone Number

Cy34.1

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Mouse CD22 Antibody, Monoclonal (Cy34.1), could be used for in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κLight Chain (clone MAR 18.5), Flow, IP and so on.
Application:
in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κLight Chain (clone MAR 18.5): Use at an assay dependent concentration. 
Flow: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration. 
Protein Function
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Spécifications

Product Name

CD22 Mouse mAb - Anticorps primaire, specific to CD22, Mouse IgG1

Synonymes

Lyb8|Lyb-8|B-cell receptor CD22|BL-CAM|B-lymphocyte cell adhesion molecule|CD22 antigenMGC130020|CD22 molecule|CD22|sialic acid binding Ig-like lectin 2|Sialic acid-binding Ig-like lectin 2|Siglec2|Siglec-2|SIGLEC2FLJ22814|T-cell surface antigen Leu-14|B cell receptor CD22 precursor antibody|B lymphocyte cell adhesion molecule antibody|B-cell receptor CD22 antibody|B-lymphocyte cell adhesion molecule antibody|BL CAM antibody|BL-CAM antibody|BLCAM antibody|CD 22 antibody|CD22 antibody|CD22 antigen antibody|CD22 molecule antibody|CD22 protein antibody|Lectin 2 antibody|Leu14 antibody|Lyb8 antibody|MGC130020 antibody|sialic acid binding Ig like lectin 2 antibody|Sialic acid binding immunoglobulin like lectin 2 antibody|Sialic acid-binding Ig-like lectin 2 antibody|SIGLEC 2 antibody|Siglec-2 antibody|SIGLEC2 antibody|T cell surface antigen Leu 14 antibody|T-cell surface antigen Leu-14 antibody

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Espèce hôte

Souris

Spécificité

CD22

Immunogène

B10.D2 mouse splenocytes

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

See COA

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at 2-8°C,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CD22 Mouse mAb (Ab094278)

CD22 Mouse mAb (Ab094278) - Flow Cytometry
Two-color flow cytometric analysis of CD22 expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Mouse IgG Isotype Control Antibody (Biotin) (Ab180306; Left panel) or CD22 Mouse mAb (Ab094278; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD22 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Application

Application

Dilution info

IP

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κLight Chain (clone MAR 18.5)

Use at an assay dependent concentration.