Mouse IgG2a-kappa (AF700), anti-A29L - Isotype Control Antibody, specific to A29L

Cat. No.: Ab216543
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
Flow Cytometry
Host species
Mouse
Reactive species
Virus
Isotype
Mouse IgG2a
Clone Number
FG07/4F10
Conjugation
AF700
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab216543-10μg
10
$119.90
25μg
Ab216543-25μg
2
$223.90
100μg
Ab216543-100μg
1
$565.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse IgG2a, Isotype Control Antibody (FG07/4F10), could be used for Flow Cytometry.

Specifications

Product Name
Mouse IgG2a-kappa (AF700), anti-A29L - Isotype Control Antibody, specific to A29L
Synonyms
Mouse IgG2a, κ Isotype Control | Purified Mouse IgG2a, kappa Isotype Control | Mouse IgG2a Isotype Control Antibody | Mouse IgG2a Isotype Control mAb | Immunoglobulin G2A Fc | Mouse IgG2a Isotype Control | Isotype control mAb for mouse IgG2a | IgG2a (Mous
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two h
Host species
Mouse
Specificity
A29L
Immunogen
Recombinant MPVX A29L Protein
Conjugation
AF700
Excitation (nm)
Red Laser(633nm)
Emission(nm)
719nm
Ex/Em(nm)
Ex:696nm;Em:719nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Mouse IgG2a-kappa (AF700), anti-A29L (Ab216543) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Mouse mAb (AF700) (Ab190492) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG2a-kappa (AF700), anti-A29L (Ab216543).

Mouse IgG2a-kappa (AF700), anti-A29L (Ab216543) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Mouse mAb (AF700) (Ab190492) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG2a-kappa (AF700), anti-A29L (Ab216543).   Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

Use at an assay dependent concentration.
Each lot of this antibody is quality control tested by flow cytometric analysis as negative control. Use at concentrations comparable to those of the speci

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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