CD272/BTLA Mouse mAb - Primary antibody, specific to CD272/BTLA, Mouse IgG1

Cat. No.: Ab094366

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Depletion, Flow Cytometry, Functional Assay

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG1

Clone Number

6F7

Conjugation

Unconjugated

Overview

Mouse anti Mouse CD272/BTLA Antibody, Monoclonal (6F7), could be used for Flow, in vivo BTLA+ B cell and CD4 T cell depletion and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo BTLA+ B cell and CD4 T cell depletion: Use at an assay dependent concentration.
Protein Function
Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.

Specifications

Product Name

CD272/BTLA Mouse mAb - Primary antibody, specific to CD272/BTLA, Mouse IgG1

Synonyms

B and T lymphocyte associated protein antibody | B and T lymphocyte attenuator antibody | B and T lymphocyte associated antibody | B- and T-lymphocyte attenuator antibody | B- and T-lymphocyte-associated protein antibody | BTLA antibody | BTLA_HUMAN antib

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Mouse

Specificity

CD272/BTLA

Immunogen

Ig domain of C57BL/6 mouse BTLA

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CD272/BTLA Mouse mAb (Ab094366)

CD272/BTLA Mouse mAb (Ab094366) - Flow Cytometry
Two-color flow cytometric analysis of CD272/BTLA expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Mouse IgG Isotype Control Antibody (Biotin) (Ab180306; Left panel) or CD272/BTLA Mouse mAb (Ab094366; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD272/BTLA (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Flow Cytometry: CD272/BTLA Mouse mAb (Ab094366)

CD272/BTLA Mouse mAb (Ab094366) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD272/BTLA with CD272/BTLA Mouse mAb (Ab094366) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150). 

Flow Cytometry: CD272/BTLA Mouse mAb (Ab094366)

CD272/BTLA Mouse mAb (Ab094366) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD272/BTLA (red) with CD272/BTLA Mouse mAb (Ab094366) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo BTLA+ B cell and CD4 T cell depletion:

  Use at an assay dependent concentration;

Associated Targets(Human)

BTLA Tbio B- and T-lymphocyte attenuator (0 Activities)

Discover Target: BTLA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)