CD32 Mouse mAb (Biotin) - Primary antibody, specific to CD32, Mouse IgG2b

Cat. No.: Ab179707

GRADE & PURITY Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2b

Clone Number

IV.3

Conjugation

Biotin

Overview

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

CD32 Mouse mAb (Biotin) - Primary antibody, specific to CD32, Mouse IgG2b

Synonyms

CD32 antigen | CD32B | CD32Fc fragment of IgG, low affinity II, receptor for (CD32) | CDw32 | Fc fragment of IgG, low affinity IIb, receptor (CD32) | Fc fragment of IgG, low affinity IIb, receptor for (CD32) | Fc gamma RII | Fc gamma RIIb | FCG2 | Fc-gamm

Specifications & Purity

Validated, 0.5 mg/mL

Host species

Mouse

Specificity

CD32

Immunogen

The FcR of K562 cells

Positive Control

Flow: Human peripheral blood monocytes

Conjugation

Biotin

Grade

Validated

Isotype

Mouse IgG2b

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD32 Mouse mAb (Biotin) (Ab179707)

CD32 Mouse mAb (Biotin) (Ab179707) - Flow Cytometry
Flow cytometry analysis of CD32 expression by Human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD32 Mouse mAb (Biotin) (Ab179707) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: CD32 Mouse mAb (Biotin) (Ab179707)

CD32 Mouse mAb (Biotin) (Ab179707) - Flow Cytometry
Flow cytometry analysis of CD32 expression by Human peripheral blood granulocytes (red) compared to an isotype control (blue). Cells were stained with CD32 Mouse mAb (Biotin) (Ab179707) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.5μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

FCGR2A Tbio Low affinity immunoglobulin gamma Fc region receptor II-a (0 Activities)

Discover Target: FCGR2A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action