GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Post-translational: Phosphorylated by CHK1 on
Espèce hôte
Souris
Spécificité
CDC25C
Immunogène
Recombinant fusion protein of human CDC25C.
Contrôle positif
WB: Hep G2, K562, MCF7, Raji and HEK293 cell lysates. IHC: Human testis tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Mouse IgG2a
Type de chaîne lumineuse
kappa
SDS-PAGE
150 kDa
Méthode de purification
Protein A purified
Concentration
0.5 mg/mL
Stockage et expédition
Forme
Liquid
Conditions de stockage de stockage
Store at -20°C,Avoid repeated freezing and thawing
Expédié en
Ice chest + Ice pads
Stabilité et stockage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
CDC25C Mouse mAb (Ab095606) - Western Blot All lanes: CDC25C Mouse mAb (Ab095606) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 53 kDa Observed band size: 55 kDa Exposure time: 12.6 s
CDC25C Mouse mAb (Ab095606) - IHC Immunohistochemical analysis of CDC25C staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with CDC25C Mouse mAb (Ab095606) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Application
Application
Dilution info
IHC
1/50-1/200
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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