CEBPA Mouse mAb - Anticorps primaire, specific to CEBPA, IgG1

Cat. No.: Ab095961

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0mg/mL

Application

ELISA, Flow Cytometry, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG1

Clone Number

4E10

Conjugation

Unconjugated

Vue d’ensemble

Mouse Monoclonal (4E10) to CEBPA , could be used for WB, Flow and ELISA.

Applications
WB：1/500 - 1/2000;
Flow：1/200 - 1/400;
ELISA ：1/10000;

Purity
Protein G purified

Function
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Specifications

Product Name

CEBPA Mouse mAb - Anticorps primaire, specific to CEBPA, IgG1

Synonymes

C/EBP alpha

Spécifications et pureté

ExactAb™, Validated, Carrier Free, 1.0mg/mL

Espèce hôte

Souris

Spécificité

CEBPA

Immunogène

Purified recombinant fragment of human CEBPA expressed in E. Coli.

Contrôle positif

WB: THP-1 cell lysate, Flow: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

1.0mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C. Avoid freeze/thaw cycle.

Images

Western Blot: CEBPA Mouse mAb (Ab095961)

CEBPA Mouse mAb (Ab095961) - Western Blot
All lanes: CEBPA Mouse mAb (Ab095961) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 42&26 kDa
Observed band size: 42&26 kDa

Flow Cytometry: CEBPA Mouse mAb (Ab095961)

CEBPA Mouse mAb (Ab095961) - Flow Cytometry
Flow cytometric analysis of HeLa cells using CEBPA Mouse mAb (Ab095961) at 1/200 dilution (green) and negative control (red).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/200-1/400 ;

• ELISA:

  1/10000 ;

Cibles associées (humaines)

CEBPA Tbio CCAAT/enhancer-binding protein alpha (0 Activities)

Discover Target: CEBPA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CEBPA Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.