Substantial equivalence (metabolomics) experiments
Modern "metabolomics" methods can be used to automatically compare the levels of a number of structurally different compounds in a large number of samples. The
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Substantial equivalence (transcriptomics) experiments
In Western Europe, for transgenic crops to be approved for commercial production, proof of their substantial equivalence to conventionally bred crops needs to b
Experiments on oat transgenesis and its application in improving osmotic stress tolerance
Oat, a temperate cereal crop widely grown worldwide, lacks tolerance to osmotic stress caused by drought and/or high salt. We have developed a genotype-independ
Experiments on the design and management of field trials of transgenic crops
With the development of transgenic technology systems, the transfer of heterologous genes into the plant genome has become possible, which provides a new avenue
Experimental methods of Agrobacterium-mediated transformation of barley
Agrobacterium-mediated transformation of barley has been widely utilized over the last decade and it offers advantages over gene gun-mediated transgenic techniq
Gene insertion site and pattern experiments
The study of insertion patterns and loci of exogenous genes in transgenic plants greatly contributes to the understanding of the mechanisms of integration, expr
Cereal plant seed protein extraction experiment
Seeds may contain different components, like starch and polysaccharides, which can severely reduce the amount of protein extracted. The proteins in cereal plant
Protein extraction experiments on woody plants
Within this chapter, we present a protocol for extracting total protein from wood-forming tissues (secondary xylem in differentiation). This protocol has been u
Plant mitochondria preparation and its substructure hierarchical separation experiments
Plant cell mitochondria perform a number of important biochemical processes. The most important of these is the oxidative degradation of organic acids via the t