Nuclear Protein Extraction Experiment
The integrity of the proteome of subcellular components such as the nucleus is largely determined by whether or not other cellular contaminants are removed from
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Extraction and solubilization experiments of plant plasma membrane proteins
In all living cells the plasma membrane (PM ) exists as an interface between the cytoplasm and the environment and is one of the most complex and differentiated
Experiments on the quantitative analysis of two-dimensional gels
Two-dimensional electrophoresis, as an important experimental tool for comparative proteomics studies, has been applied to conveniently reflect the differences
Nanoscale two-dimensional liquid chromatography tandem mass spectrometry of rice leaf and root proteins
In this chapter, we present a detailed experimental method for the large-scale identification of proteins in rice leaf and root tissues using 2D liquid chromato
Peptide mass fingerprinting and protein identification by MALDI-TOF method
MALDI-TOF peptide mass fingerprinting (PMF) is a faster and more convenient method for identifying proteins. The genomes studied have been sequenced and annotat
Protein Edman sequencing experiments on 2D gels
Immunoblotting using polyvinylidene difluoride (PVDF ) membranes is the most commonly used method for Edman sequencing. Protein samples are transferred from a 2
Isolation and characterization of membrane proteins and their functional analysis-GFC/IEC/SDS-PAGE and MALDI-TOF-MS experiments
Prior to the identification of membrane proteins by matrix-assisted laser-resolved time-of-flight mass spectrometry (MALDI-TOF-MS), these proteins need to be se
2-DE PROTICdb database experiment for proteomics
PROTICdb is a web-based database designed primarily to store and analyze plant proteomic data obtained by 2D polyacrylamide electrophoresis and mass spectrometr
Plant proteomics and glycosylation experiments
Like other eukaryotic cells, glycosylation is one of the most studied post-translational events in plant cells. Depending on the type of connection between the