denaturing gradient gel electrophoresis
Denaturing gradient gel electrophoresis (DGGE) is a gel system that enables the separation of DNA fragments according to their melting properties. (1) By settin
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Determination of the amount of DNA fixed in ethanol
Determination of DNA content can be used to (1) perform gene fragment ligation experiments. (2) Perform plasmid concentration determination prior to transformat
Pulsed field gel electrophoresis experiment
Pulsed-field gel electrophoresis, which alternates periodically between two differently oriented electric fields, allows the separation of DNA molecules up to 5
Stability screening assay for transfected cells
This experiment is mainly used to obtain eukaryotic cells containing the target exogenous gene.
Ligation, transformation and screening of recombinant plasmids
Recombinant plasmid ligation, transformation and screening are mainly used to obtain recombinants containing the target gene linkage.
Detection of methylation by sequencing after bisulfite modification
Bisulfite-modified sequencing can be used primarily to detect methylation.
Expression experiments of multidrug resistance genes
Expression experiments of multidrug resistance genes are mainly used for tumor therapy research.
Amplification experiments of phage libraries
The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
Sieving agarose gel electrophoresis experiments
Sieve agarose gels spread and electrophoresis like regular agarose gels, but they can resolve small fragments of DNA as well as non-denaturing polyacrylamide ge