Expression experiments of multidrug resistance genes
Expression experiments of multidrug resistance genes are mainly used for tumor therapy research.
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Ligation, transformation and screening of recombinant plasmids
Recombinant plasmid ligation, transformation and screening are mainly used to obtain recombinants containing the target gene linkage.
Oligonucleotide-mediated mutagenesis experiments
The mutated oligonucleotide is used to direct the synthesis of the template, thereby altering the DNA sequence, with a mutation efficiency of 50-80%.
Plate laying and transfer experiments of phage libraries
The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
Purification experiments for mucoid and plasmid clones
The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
Gene Synthesis Experiment
Gene synthesis can be applied to (1) metabolic pathway synthesis, (2) gene network construction, and (3) vaccine design.
merged oligonucleotide mutagenesis assay
An important feature of this method is the direct cloning of single-stranded, abbreviated oligonucleotides into homologous double-stranded molecules after their
Plate-laying and transfer experiments for stickies and plasmid libraries
The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
Region-specific mutagenesis experiments
Region-specific mutagenesis experiments for (1) gene-induced modification (2) specific expression of genes (3) clinical anti-tumor and other targets.