Culture of colorectal tumor cells
The adenoma is usually digested with enzymes, and the cancerous tissue is minced with a scalpel blade. If well-differentiated colorectal cancer specimens are no
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Primary culture of adipocytes
This experiment is mainly used for primary culture of adipocytes.
Long-term culture of bone marrow hematopoietic cells
Bone marrow is aspirated into the culture medium. The bone marrow is maintained as an attached multilayer of cells for at least 12 weeks and up to 30 weeks. Ste
Sterilization of filtration units
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Alginate coating
Cells in 75 cm2 culture flasks were digested with trypsin, counted and mixed with alginate solution. Alginate cell suspension was added dropwise to calcium chlo
Hematopoietic cell colony formation assay
Bone marrow cells were suspended with agar or methylcellulose, and then the cells were inoculated in Petri dishes with appropriate growth factors.
Separation of mouse embryos
Isolation of mouse embryos can be used for: (1) Culturing mouse embryos in vitro and figuring out appropriate culture conditions. (2) Using embryos for developm
density gradient cell separation method
This experiment is mainly used to isolate cells.
Osteoblast primary culture experiment
This experiment was used for primary culture of osteoblasts.