Report gene: in situ staining assay for beta-galactosidase
In situ staining for β-galactosidase can be applied (1) to determine whether the guide DNA has been integrated into the host cell and (2) to trace cell-cell in
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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rotary flask culture (chemistry)
Add the cell suspension to a round culture flask and then place the flask on a rotating rack and turn it slowly.
nerve aggregates
Brains were removed from fetal rats at 17 d or 18 d of gestation (contact the local animal ethics committee) and the brains were made into single cell suspensio
Stable transfection by electroporation
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
microcarrier
Cells and microbeads were inoculated at high concentrations and then diluted, stirred and sampled as required.
Petri dish
Cells were inoculated into filter membrane Petri dishes and cultured in excess culture solution in multiwell culture plates.
Inoculation of suspension cells in cell culture flasks
Inoculation of suspension cells in cell culture flasks can be used for (1) mass culture of cells and (2) providing cells with a suitable environment for growth.
Isolation and culture of mouse keratinocytes
This protocol is a modification of the force method of Hennings et al. (1980). Gestational BALB/c mice are available from Harland Sprague Dawley, and the protoc
Trypsin treatment of monolayer cells
The following protocol describes the use of trypsin to treat cells for passaging or collection. Cell lines are usually maintained by passaging once a week and c