Basic techniques for the preparation and use of PPA
Cross-linking of the initially purified SPA with HRP by sodium periodate oxidation (cross-linking reaction time at 22°C for 5h) gives PPA.
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MARK signaling pathway
The mitogen-activated protein kinase (MAPK) signaling pathway is a signaling pathway that is widely present in various cells and consists of a set of cascade-ac
Construction of siRNA expression vector targeting EGFR gene
The constructed psj-EGFR vector was transfected into A549 cells using Lipofeetamine2000, and real-time quantitative fluorescence PCR, immunofluorescence and flo
PCR-ELISA Telomerase Assay
Telomeres are specific DNA-protein structures at the ends of eukaryotic chromosomes, and telomeric DNA is a series of repetitive G-rich DNA sequences, which are
Transcription cDNA probe
A probe that has obtained mRNA from the transcription of the corresponding gene, which is then obtained by reverse transcription, is called a cDNa probe (cDNa p
Rapid extraction of polysaccharide and polyphenol plant RNA
The reason why some plants are more difficult to extract high-quality RNA is related to a number of components enriched in these tissues, commonly phenolic comp
Measurement of Ph in polluted water
Food factories require that the pH value of wastewater discharged be between 6.5 and 8.5. In the production process of fruit and vegetable sweat drink factories
Methylation PCR
Methylation-Specific PCR (MS-PCR) is a new method developed by Herman et al. (1996) based on the use of bisulfite treatment. This method is highly sensitive and
G protein-coupled receptor pathway: GC-cGMP-PKG
Extracellular signals are received by receptors and delivered to AC via Gs or Gi to activate or inhibit it; AC is activated to produce cAMP, which activates PKA