3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Brain Heart Infusion Broth without Dextrose is a modification of the media described by Rosenow and Hayden in which infusion from calf brains has replaced the brain tissue and disodium phosphate has replaced the calcium carbonate buffer. The addition of 0.1% agar can be used to lower oxygen ...
Brilliant Green Agar was first described by Kristensen et al. in 1925 and later modified by Kauffman in 1933. It is classified by the USP as a highly selective medium for the recovery of Salmonella species except for tyhphoid and paratyphoid strains. Brilliant Green dye inhibits Gram positive ...
A selective medium for isolation of gram positive bacteria. Most often utilized with blood added. Eliner, et al. added colistin and nalidixic acid to Columbia Agar to suppress the growth of Gram negative bacteria. The addition of 5-10% sheep red blood cells to the base demonstrates hemolytic ...
Brilliant Green 2% Bile Broth is used to confirm the presence of coliforms. Oxbile and Brilliant Green inhibit Gram positive bacteria and many Gram negative bacteria other than coliforms. Lactose is a carbohydrate source. Bacteria that ferment lactose and produce gas are detected. This medium is ...
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