posted this at Jun 9, 2025 Updated at Jul 27, 2023
Directions per Liter:
Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
Agar
20
Lactose
10
Sucrose
10
Pancreatic Digest of Casein
5
Peptic Digest of Animal Tissue
5
Sodium Chloride
5
Yeast Extract
3
Phenol Red
0.08
Sulfadiazine
0.08
Brilliant Green
0.0125
Total: 58.1725g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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