Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Amino acid surfactants are a class of surfactants that use amino acids or amino acid-derived structures as hydrophilic groups and typically introduce hydrophobic chains through fatty acyl groups. In personal care cleansing products, the most common types are N-acyl amino acid salts. Typical ...
In the preparation of peptides and amino acid derivatives, the Fmoc (9-fluorenylmethoxycarbonyl) protection strategy is already highly mature. Merrifield established the basic framework of solid-phase peptide synthesis (SPPS), and Carpino subsequently introduced the Fmoc protecting group, which ...
Amino acid analysis is a fundamental technical component in biological sample analysis, nutritional evaluation, metabolism research, and fermentation process control. Its analytical targets include free amino acids, total amino acids after hydrolysis, specific single amino acids, and certain ...
Somatostatin is an important regulatory peptide, widely distributed in the central nervous system, endocrine glands, and gastrointestinal tract.
L-Tyrosine is a crucial non-essential amino acid widely used in cell culture and various laboratory research applications. As a fundamental component of protein synthesis, L-Tyrosine not only participates in the synthesis of various biomolecules but also plays a vital role in cell development ...
Glutamate and γ-aminobutyric acid (GABA) are two of the most important neurotransmitters in the brain. Glutamate acts as the primary excitatory neurotransmitter, playing a crucial role in signal transmission within the central nervous system, while GABA serves as the main inhibitory ...
Solid-phase peptide synthesis (SPPS) is a revolutionary biochemical technique that has become the primary method for synthesizing peptides and proteins since its introduction in the 1960s. Robert Bruce Merrifield first proposed the concept of SPPS in 1963, and this groundbreaking work not only ...
Cell culture medium is an indispensable part of cell biology research, which provides essential nutrients and a suitable develope environment for cells. The following is a comprehensive introduction to the detailed components and characteristics of several commonly used cell culture media:
Techniques often explored alongside immunological experiments.
