Hsp27 Mouse mAb - Anticorps primaire, specific to Hsp27

Cat. No.: Ab210504

GRADE & PURITY Animal Free, Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG2b

Clone Number

CD04/1E2

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human Hsp27 Antibody, clone CD04/1E2, could be used for WB, Flow Cytometry, ELISA and so on.

Specifications

Product Name

Hsp27 Mouse mAb - Anticorps primaire, specific to Hsp27

Synonymes

Heat shock 27kDa protein antibody | 28 kDa heat shock protein antibody | CMT2F antibody | DKFZp586P1322 antibody | epididymis secretory protein Li 102 antibody | Estrogen regulated 24 kDa protein antibody | Estrogen-regulated 24 kDa protein antibody | Hea

Spécifications et pureté

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mécanismes biochimiques et physiologiques

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerou

Espèce hôte

Souris

Spécificité

Hsp27

Immunogène

Recombinant protein corresponding to Human Hsp27

Contrôle positif

WB: MCF-7, HeLa, K562, A431, A549 and LNCaP whole cell lysates Flow Cytometry: HeLa cells

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Type de chaîne lumineuse

kappa

SDS-PAGE

150.0 KDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Stockage et expédition

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Western Blot: Hsp27 Mouse mAb (Ab210504)

Hsp27 Mouse mAb (Ab210504) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab210504) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 1.7 s 

Western Blot: Hsp27 Mouse mAb (Ab210504)

Hsp27 Mouse mAb (Ab210504) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab210504) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 26 kDa
Exposure time: 2.8 s 

Flow Cytometry: Hsp27 Mouse mAb (Ab210504)

Hsp27 Mouse mAb (Ab210504) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with Hsp27 Mouse mAb (Ab210504) (Red) at 0.125 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).​

Application

• Application: Dilution info
• WB:

  1/1000 - 1/20000 ;

• Flow Cytometry:

  1/1000 - 1/100,000

Cibles associées (humaines)

HSPB1 Tchem Heat shock protein beta-1 (0 Activities)

Discover Target: HSPB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Hsp27 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.