HspBP1 Mouse mAb - Anticorps primaire, specific to HspBP1

Cat. No.: Ab108976

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IHC, WB

Host species

Souris

Reactive species

conseil, Humain, Monkey, Souris

Isotype

Mouse IgG1

Clone Number

OTI1D5

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human HspBP1 Antibody, Monoclonal (OTI1D5), could be used for WB, IHC, Flow Cytometry and so on.

Specifications

Product Name

HspBP1 Mouse mAb - Anticorps primaire, specific to HspBP1

Synonymes

1500019G21Rik | FES1 | Heat shock protein 70 binding protein | Heat shock protein 70 interacting protein | Heat shock protein binding protein 1 | Heat shock protein-binding protein 1 | Heat-shock 70-KD protein-binding protein 1 | HPBP1_HUMAN | Hsp 70 bind

Spécifications et pureté

ExactAb™, Validated, See COA

Mécanismes biochimiques et physiologiques

Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.

Espèce hôte

Souris

Spécificité

HspBP1

Immunogène

Recombinant full length protein of human HspBP1 produced in HEK293T.

Contrôle positif

WB: Hep G2, HeLa, A549, Jurkat and MCF7 cell lysates. IHC: Human carcinoma of lung, ovary, pancreas, endometrium, adenocarcinoma of endometrium, carcinoma of bladder, lymphoma tissues. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY HspBP1.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - Western Blot
All lanes: HspBP1 Mouse mAb (Ab108976) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 38 kDa
Exposure time: 11.4 s

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human lung tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human ovary tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Human endometrium tissue within the normal limits using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human endometrium tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human bladder tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Human lymphoma tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

Flow Cytometry: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by HspBP1 Mouse mAb (Ab108976), and then analyzed by flow cytometry.

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/100;

• IHC:

  1/150

Cibles associées (humaines)

HSPBP1 Tbio Hsp70-binding protein 1 (0 Activities)

Discover Target: HSPBP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HspBP1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.