iNOS Mouse mAb - Anticorps primaire, specific to Human iNOS

Cat. No.: Ab169216

GRADE & PURITY Animal Free, Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG1

Clone Number

AB01/2H7

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human iNOS Antibody, clone AB01/2H7, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name

iNOS Mouse mAb - Anticorps primaire, specific to Human iNOS

Synonymes

HEP-NOS antibody | Hepatocyte NOS antibody | HEPNOS antibody | inducible antibody | Inducible nitric oxide synthase antibody | Inducible NO synthase antibody | Inducible NOS antibody | iNOS antibody | MAC NOS antibody | Macrophage NOS antibody | Nitric ox

Spécifications et pureté

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mécanismes biochimiques et physiologiques

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic targ

Espèce hôte

Souris

Spécificité

Human iNOS

Immunogène

Recombinant protein corresponding to Human iNOS

Contrôle positif

WB: Human serum Flow Cytometry: A549 cells IF/ICC: MCF-7 cells

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Type de chaîne lumineuse

kappa

SDS-PAGE

150.0 KDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Stockage et expédition

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Western Blot: iNOS Mouse mAb (Ab169216)

iNOS Mouse mAb (Ab169216) - Western Blot
All lanes: iNOS Mouse Antibody (Ab169216) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 141 kDa
Exposure time: 30.0 s

Flow Cytometry: iNOS Mouse mAb (Ab169216)

iNOS Mouse mAb (Ab169216) - Flow Cytometry 
Flow Cytometry analysis of A549 cells stained with iNOS Mouse mAb (Ab169216) (Red) at 5.0 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).​

ICC/IF: iNOS Mouse mAb (Ab169216)

iNOS Mouse mAb (Ab169216) - ICC/IF
IF analysis of iNOS (Green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with iNOS Mouse mAb (Ab169216) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

• Application: Dilution info
• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/200;

• Flow Cytometry:

  1/200-1/400 ;

Cibles associées (humaines)

NOS2 Tchem Nitric oxide synthase, inducible (0 Activities)

Discover Target: NOS2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

NOS2 Tchem Nitric oxide synthase, inducible (1636 Activities)

Discover Target: NOS2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action