Ku80 Mouse mAb - Anticorps primaire, specific to XRCC5, Mouse IgG1

Cat. No.: Ab112438

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, IHC, WB

Host species

Souris

Reactive species

Humain, Souris

Isotype

Mouse IgG1

Clone Number

5C5

Conjugation

Unconjugated

Vue d’ensemble

Application:
WB: 1/500 - 1/2000. Detects a band of approximately 86 kDa (predicted molecular weight: 83 kDa).
IHC: 1/200 - 1/1000
IF/ICC: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Specifications

Product Name

Ku80 Mouse mAb - Anticorps primaire, specific to XRCC5, Mouse IgG1

Synonymes

86 kDa subunit of Ku antigen | ATP-dependent DNA helicase 2 subunit 2 | ATP-dependent DNA helicase II 80 kDa subunit | CTC box-binding factor 85 kDa subunit | CTC85 | CTCBF | DNA repair protein XRCC5 | EC 3.6.4.- | Ku80 | Ku86 | Lupus Ku autoantigen prote

Spécifications et pureté

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Espèce hôte

Souris

Spécificité

XRCC5

Immunogène

Purified recombinant fragment of human XRCC5 (corresponding to amino acids 61-340 of Human Ku80) expressed in E. Coli

Contrôle positif

WB: A549, HeLa, HEK293, K562 lysates. ICC/IF: HeLa cells. IHC: Human tonsil tissues, Human colon cancer tissue. Flow: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

1.0 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid repeated freeze / thaw cycles.

Images

Western Blot: Ku80 Mouse mAb (Ab112438)

Ku80 Mouse mAb (Ab112438) - Western Blot
All lanes: Ku80 Mouse mAb (Ab112438) at 1/1000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 83 kDa
Observed band size: 83 kDa

ICC/IF: Ku80 Mouse mAb (Ab112438)

Ku80 Mouse mAb (Ab112438) - ICC/IF
IF analysis of HeLa cells using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 (green). Red: Actin filaments have been labeled with AF555 phalloidin.

IHC: Ku80 Mouse mAb (Ab112438)

Ku80 Mouse mAb (Ab112438) - IHC
Immunohistochemical analysis of paraffin-embedded Human tonsil tissues using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 with DAB staining.

IHC: Ku80 Mouse mAb (Ab112438)

Ku80 Mouse mAb (Ab112438) - IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 with DAB staining.

Flow Cytometry: Ku80 Mouse mAb (Ab112438)

Ku80 Mouse mAb (Ab112438) - Flow Cytometry
Flow cytometric analysis of HeLa cells using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 (green) and negative control (purple).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IF/ICC:

  1/200-1/1000 ;

• Flow Cytometry:

  1/200-1/400 ;

• ELISA:

  1/10000 ;

• IHC:

  1/200-1/1000 ;

Cibles associées (humaines)

XRCC5 Tbio X-ray repair cross-complementing protein 5 (0 Activities)

Discover Target: XRCC5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Ku80 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.