MUC1/CD227 Mouse mAb - Anticorps primaire, specific to MUC1/CD227, Mouse IgG3

Cat. No.: Ab116379

QUALITÉ & PURETÉ Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Flow Cytometry, Functional Assay, IF/ICC, IHC, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG3

Clone Number

C595 (NCRC48)

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human MUC1/CD227 Antibody, Monoclonal (C595 (NCRC48)), could be used for in vivo administration in mouse xenograft models, IHC, ICC, IF, in vitro cell cytotoxicity assay, WB, Flow and so on.
Application:
in vivo administration in mouse xenograft models: Use at an assay dependent concentration. 
IHC: Use at an assay dependent concentration. 
ICC/IF: Use at an assay dependent concentration. 
in vitro cell cytotoxicity assay: Use at an assay dependent concentration. 
WB: Use at an assay dependent concentration. 
Flow: Use at an assay dependent concentration.
Protein Function
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Spécifications

Product Name

MUC1/CD227 Mouse mAb - Anticorps primaire, specific to MUC1/CD227, Mouse IgG3

Synonymes

MCD | PUM | MCKD | MCKD1 | ADMCKD | ADTKD2 | Ca15-3 | ADMCKD1 | CA 15-3 | MUC-1/X | MUC-1/SEC | Breast carcinoma-associated antigen DF3 | Carcinoma-associated mucin | CD227 antigen | CD227 | DF3 antigen | EMA | Episialin | H23 antigen | H23AG | KL-6 | MAM

Spécifications et pureté

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Espèce hôte

Souris

Spécificité

MUC1/CD227

Immunogène

Purified human MUC1

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Mouse IgG3

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

See COA

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at 2-8°C,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: MUC1/CD227 Mouse mAb (Ab116379)

MUC1/CD227 Mouse mAb (Ab116379) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling MUC1/CD227 (red) with MUC1/CD227 Mouse mAb (Ab116379) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application

Dilution info

WB

Use at an assay dependent concentration.

IHC

Use at an assay dependent concentration.

IF/ICC

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo administration in mouse xenograft models

Use at an assay dependent concentration. 

in vitro cell cytotoxicity assay

Use at an assay dependent concentration. 

Cibles associées (humaines)

MUC1 Tchem Mucin-1 (0 Activities)

Discover Target: MUC1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MUC1/CD227 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.