OBFC1 Mouse mAb - Anticorps primaire, specific to STN1

Cat. No.: Ab119117

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG1

Clone Number

OTI2E4

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human OBFC1 Antibody, Monoclonal (OTI2E4), could be used for WB, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

OBFC1 Mouse mAb - Anticorps primaire, specific to STN1

Synonymes

AAF 44 | AAF44 | Alpha accessory factor 44 | bA541N10.2 | CST complex subunit STN1 | FLJ22559 | Obfc1 | Oligonucleotide/oligosaccharide binding fold containing 1 | Oligonucleotide/oligosaccharide-binding fold-containing protein 1 | OTTHUMP00000020417 | OT

Spécifications et pureté

ExactAb™, Validated, See COA

Mécanismes biochimiques et physiologiques

Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bin

Espèce hôte

Souris

Spécificité

STN1

Immunogène

Recombinant protein of human OBFC1.

Contrôle positif

WB: Hep G2, A549, Jurkat and MCF7 celllysates. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY OBFC. Flow Cytometry: HEK293T transfected with pCMV6-ENTRY OBFC1.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: OBFC1 Mouse mAb (Ab119117)

OBFC1 Mouse mAb (Ab119117) - Western Blot
All lanes: OBFC1 Mouse mAb (Ab119117) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 100.0 s

IF/ICC: OBFC1 Mouse mAb (Ab119117)

OBFC1 Mouse mAb (Ab119117) - IF/ICC
OBFC1 Mouse mAb (Ab119117) immunofluorescent staining of COS7cells transiently transfected by pCMV6-ENTRYOBFC1.

Flow Cytometry​: OBFC1 Mouse mAb (Ab119117)

OBFC1 Mouse mAb (Ab119117) - Flow Cytometry​
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by OBFC1 Mouse mAb (Ab119117) , and then analyzed by flow cytometry.

Application

• Application: Dilution info
• WB:

  1/2000;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

Cibles associées (humaines)

STN1 Tbio CST complex subunit STN1 (0 Activities)

Discover Target: STN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (OBFC1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.