PCNA Mouse mAb - Anticorps primaire, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to PCNA, IgG1

Cat. No.: Ab155826

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, See COA

Application

Flow Cytometry, IF/ICC, WB

Host species

Souris

Reactive species

conseil, Humain, Souris

Isotype

Mouse IgG1

Clone Number

C04/3D7

Conjugation

Unconjugated

Vue d’ensemble

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

Specifications

Product Name

PCNA Mouse mAb - Anticorps primaire, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to PCNA, IgG1

Synonymes

Cyclin | PCNA

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA

Espèce hôte

Mouse

Espèce hôte

Souris

Spécificité

PCNA

Application

WB,Flow,ELISA,ICC/IF

Immunogène

Full length recombinant human PCNA

Contrôle positif

WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Type d'anticorps

Primary antibody

Clonalité

Monoclonal antibodies

Isotype

IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Source

Hybridoma supernatant

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze/thaw cycle.

Images

Western Blot: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

ICC/IF : PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Ab155826) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Flow Cytometry
Flow analysis of PCNA (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. PCNA Mouse mAb (Ab155826) at 1/4000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Western Blot: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Antibody (Ab155826) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 29.0 kDa
Observed band size: 32.0 kDa
Exposure time: 97.0 s 

Application

• Application: Dilution info
• IF/ICC:

  0.5 μg/mL ;

• Flow Cytometry:

  0.016-2.0μg/mL ;

• WB:

  1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa. ;

Cibles associées (humaines)

PCNA Tchem Proliferating cell nuclear antigen (0 Activities)

Discover Target: PCNA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action