Ponceau S, also named Tetrasodium 3-hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonate, has the molecular formula C₂₂H₁₂N₄Na₄O₁₃S₄, a molecular weight of 760.5688 and CAS No. 6226-79-5. It is a water-soluble anionic diazo dye synthesized by the coupling of diazoaminoazobenzene disulfonic acid and naphthol disulfonic acid into its tetrasodium salt. Negatively charged Ponceau S can bind to positively charged amino acid residues as well as the non-polar regions of proteins, producing distinct red protein bands. Protein staining with Ponceau S is reversible; the stain can be washed off with distilled water, PBS or other appropriate buffer solutions. This staining reagent is applicable for protein detection on PVDF membranes (Western blots), nitrocellulose membranes (NC) and cellulose acetate membranes, but is not compatible with nylon membranes.Ponceau S Staining Elution Solution, also referred to as destaining solution, is mainly composed of sodium hydroxide (NaOH) and used to elute Ponceau S stain from membranes. This product is for research use only and shall not be used for other purposes.
Storage Conditions:
Store at room temperature, valid for one year.
Operating Procedures (For Reference Only):
1. Operate in accordance with the specific requirements of the experiment.
Precautions:
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is only for scientific research and experimental use. It is strictly prohibited to use it for clinical diagnosis, human injection, food addition, cosmetic production and other non-research scenarios.
3. Users shall be responsible for their own personal protection and laboratory safety management. Any personal injury, equipment damage, environmental pollution and other consequences caused by improper operation or failure to use the reagent in accordance with the protection requirements shall be borne by the user.
