SIL1 Mouse mAb - Anticorps primaire, specific to SIL1

Cat. No.: Ab127610

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Souris

Reactive species

conseil, Humain, Souris

Isotype

Mouse IgG1

Clone Number

OTI3E3

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human SIL1 Antibody, Monoclonal (OTI3E3), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

SIL1 Mouse mAb - Anticorps primaire, specific to SIL1

Synonymes

BAP | BiP associated protein | BiP-associated protein | Endoplasmic reticulum chaperone SIL 1 | Endoplasmic reticulum chaperone SIL1 | MSS | Nucleotide exchange factor SIL 1 | Nucleotide exchange factor SIL1 | SIL 1 | sil1 | SIL1 homolog | SIL1 homolog en

Spécifications et pureté

ExactAb™, Validated, See COA

Mécanismes biochimiques et physiologiques

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.

Espèce hôte

Souris

Spécificité

SIL1

Immunogène

Recombinant protein of human SIL1.

Contrôle positif

WB: Hep G2, HeLa, A549 and A431 cell lysates. IHC: Human kidney, liver, carcinoma of liver, adenocarcinoma of ovary tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY SIL1, HT29 cells. Flow Cytometry: HEK293T transfected with pCMV6-ENTRY SIL1.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Méthode de purification

Protein G purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - Western Blot
All lanes: SIL1 Mouse mAb (Ab127610) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 48, 54 kDa
The expression profile and molecular weight observed, which is due to glycosylation.
Exposure time: 57.2 s

IHC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)

IHC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)

IHC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab127610, Dilution 1/50)

IHC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using SIL1 Mouse mAb (Ab127610). (Heatinduced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)

IF/ICC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IF/ICC
SIL1 Mouse mAb (Ab127610) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.

IF/ICC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IF/ICC
Immunofluorescent staining of HT29 cells using SIL1 Mouse mAb (Ab127610).

Flow Cytometry: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY SIL1 (Red) or empty vector control plasmid (Blue) were immunostained with SIL1 Mouse mAb (Ab127610), and then analyzed by flow cytometry.

Application

• Application: Dilution info
• IHC:

  1/100-1/500;

• IF/ICC:

  1/100;

• WB:

  1/200-1/1000;

• Flow Cytometry:

  1mg/ml;

Cibles associées (humaines)

SIL1 Tbio Nucleotide exchange factor SIL1 (0 Activities)

Discover Target: SIL1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)