TAGLN Mouse mAb - Anticorps primaire, specific to TAGLN, IgG2b

Cat. No.: Ab156196

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Souris

Reactive species

conseil, Humain, Souris

Isotype

Mouse IgG2b

Clone Number

AB04/4H10

Conjugation

Unconjugated

Vue d’ensemble

Application
WB: 1/1000-1/100,000; Predicted molecular weight: 22.6 kDa
Flow: 0.016 μg/mL – 1 μg/mL
ICC/IF: 5 μg/mL

Specifications

Product Name

TAGLN Mouse mAb - Anticorps primaire, specific to TAGLN, IgG2b

Synonymes

22 kDa actin-binding protein antibody | Protein WS3-10 antibody | SM22 antibody | SM22-alpha antibody | Smooth muscle protein 22-alpha antibody | TAGL_HUMAN antibody | TAGLN antibody | Transgelin antibody | WS3-10 antibody | Anti-TAGLN mAb | Transgelin va

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL

Espèce hôte

Souris

Spécificité

TAGLN

Immunogène

Recombinant full length protein corresponding to Human Transgelin

Contrôle positif

WB: HeLa, A549, A431, Raji, NIH/3T3, C6 Flow: HeLa cells ICC/IF: HeLa cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotype

IgG2b

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: TAGLN Mouse mAb (Ab156196)

TAGLN Mouse mAb (Ab156196) - Western Blot
All lanes: TAGLN Antibody (Ab156196) at 1.0 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 22.6 kDa
Observed band size: 23.6 kDa
Exposure time: 47.1 s 

Flow Cytometry: TAGLN Mouse mAb (Ab156196)

TAGLN Mouse mAb (Ab156196) - Flow Cytometry
Overlay histogram showing HeLa cells stained with TAGLN Mouse mAb (Ab156196) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab179003) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue)

ICC/IF: TAGLN Mouse mAb (Ab156196)

TAGLN Mouse mAb (Ab156196) - ICC/IF
IF analysis of TAGLN (Red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with TAGLN Mouse mAb (Ab156196) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

• Application: Dilution info
• Flow Cytometry:

  0.016 μg/mL- 1 μg/mL;

• IF/ICC:

  5.0 μg/mL;

• WB:

  1/1000-1/100,000; Predicted molecular weight: 22.6 kDa;

Cibles associées (humaines)

TAGLN Tbio Transgelin (0 Activities)

Discover Target: TAGLN

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TAGLN Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.