Vinculin Mouse mAb - Anticorps primaire, specific to Vinculin, IgG1

Cat. No.: Ab179928
DISPONIBLE À COMMANDE
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. 1.0 mg/mL
Application
Flow Cytometry, WB
Host species
Souris
Reactive species
Humain, Souris
Isotype
Mouse IgG1
Clone Number
AB04/5D4
Conjugation
Unconjugated
Size
Allemagne (EU)
USA*
Price
Qty
10μl
Ab179928-10μl
En stock ≥10
43,30€
50μl
Ab179928-50μl
En stock ≥10

119,66€

173,46€
Enregistrer 53,80 € (31.02%)
100μl
Ab179928-100μl
En stock ≥10

184,74€

260,23€
Enregistrer 75,49 € (29.01%)
1ml
Ab179928-1ml
1 En stock
1 301,52€
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Vue d’ensemble

Application
WB: 1/1,000-1/20,000 ; Predicted molecular weight: 124 kDa
Flow: 0.125 μg/mL-1.0 μg/mL

Specifications

Product Name
Vinculin Mouse mAb - Anticorps primaire, specific to Vinculin, IgG1
Synonymes
CMD1W antibody | CMH15 antibody | Epididymis luminal protein 114 antibody | HEL114 antibody | Metavinculin antibody | MV antibody | MVCL antibody | OTTHUMP00000019861 antibody | OTTHUMP00000019862 antibody | VCL antibody | VINC antibody | VINC_HUMAN antib
Spécifications et pureté
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL
Espèce hôte
Souris
Spécificité
Vinculin
Immunogène
Recombinant full length protein corresponding to Human Vinculin
Contrôle positif
WB: HeLa, HEK293, HepG2, A431 and NIH/3T3 whole cell lysates Flow: HeLa cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotype
IgG1
Type de chaîne lumineuse
kappa
SDS-PAGE
150 kDa
Méthode de purification
Protein A purified
Concentration
1.0 mg/mL
Stockage et expédition
Forme
Liquid
Conditions de stockage de stockage
Store at -20°C,Avoid repeated freezing and thawing
Expédié en
Ice chest + Ice pads
Stabilité et stockage
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Vinculin Mouse mAb (Ab179928) - Western Blot
All lanes: Vinculin Antibody (Ab179928) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124.0 kDa
Observed band size: 129.7 kDa
Exposure time: 5.7 s 

Vinculin Mouse mAb (Ab179928) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab179928) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Application
ApplicationDilution info
WB

1/1,000-1/20,000 ; Predicted molecular weight: 124 kDa

Flow Cytometry

0.125 μg/mL-1.0 μg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Cibles associées (humaines)
VCL Tbio Vinculin (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mécanismes d'action
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateArticle
ZJ24F0202971Certificate of AnalysisMar 01, 2024 Ab179928
ZJ24F0202970Certificate of AnalysisMar 01, 2024 Ab179928
ZJ24F0202969Certificate of AnalysisMar 01, 2024 Ab179928
ZJ24F0202968Certificate of AnalysisMar 01, 2024 Ab179928
FAQ et articles
Calculateurs de solution
Avis

Avis des clients

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

Produits apparentés

Goat Anti-Mouse IgG H&L Antibody - Anticorps secondaire, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - Anticorps secondaire, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - Anticorps de contrôle isotype, generated in Rat, >95%, IgG
Rabbit Anti-Mouse IgG H&L (HRP) - Anticorps secondaire, IgG
BALB/c Mouse IgG - Anticorps de contrôle isotype, IgG
Rabbit Anti-Mouse IgG antibody - Anticorps secondaire
Goat Anti-Mouse IgG H&L (FITC) - Anticorps secondaire
Goat Anti-Mouse IgG H&L (TRITC) - Anticorps secondaire
Goat Anti-Mouse IgG H&L Antibody - Anticorps secondaire, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Anticorps secondaire
Goat Anti-Mouse IgG H&L (HRP) - Anticorps secondaire
Goat Anti-Mouse IgG H&L (Biotin) - Anticorps secondaire
Recombinant Vinculin Antibody - Anticorps primaire, specific to Vinculin, Rabbit IgG
Rabbit Anti-Mouse IgG (HRP) - Anticorps secondaire
Rabbit Anti-Mouse IgG (Biotin) - Anticorps secondaire
Vinculin Mouse mAb - Anticorps primaire, specific to Vinculin, IgG1
Goat Anti-Mouse IgG H&L (HRP) - Anticorps secondaire, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - Anticorps secondaire, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - Anticorps secondaire, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - Anticorps secondaire, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Anticorps secondaire, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - Anticorps secondaire, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - Anticorps secondaire, Goat IgG
Mouse IgG (Biotin) - Anticorps de contrôle isotype, Mouse IgG
Mouse IgG (PE) - Anticorps de contrôle isotype, Mouse IgG
Mouse IgG (APC) - Anticorps de contrôle isotype, Mouse IgG
Mouse IgG (FITC) - Anticorps de contrôle isotype, Mouse IgG
Mouse IgG2a-Fc - Anticorps de contrôle isotype, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - Anticorps secondaire, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - Anticorps secondaire, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - Anticorps secondaire, Goat IgG
Mouse IgG2b-kappa, anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - Anticorps de contrôle isotype, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - Anticorps de contrôle isotype, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - Anticorps de contrôle isotype, specific to HEL, Mouse IgG2a(D265A)
Mouse IgG1(D265A)-kappa, anti-HEL - Anticorps de contrôle isotype, specific to HEL, Mouse IgG1(D265A)
Mouse IgG (AF488) - Anticorps de contrôle isotype, IgG
Mouse IgG (PerCP) - Anticorps de contrôle isotype, IgG
Mouse IgG (AF405) - Anticorps de contrôle isotype, IgG
Mouse IgG (AF647) - Anticorps de contrôle isotype, IgG
Goat Anti-Mouse IgG H&L (AF555) - Anticorps secondaire, specific to Mouse IgG, Goat IgG
Mouse IgG (AF700) - Anticorps de contrôle isotype, IgG
Goat Anti-Mouse IgG H&L (AF700) - Anticorps secondaire, specific to Mouse IgG, Goat IgG
Mouse IgG (PE-Cy5) - Anticorps de contrôle isotype
Mouse IgG (PE-Cy7) - Anticorps de contrôle isotype
Mouse IgG2b-kappa, anti-E8L - Anticorps de contrôle isotype, specific to E8L
Mouse IgG2a-kappa, anti-A29L - Anticorps de contrôle isotype, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - Anticorps de contrôle isotype
Mouse IgG2a-Fc (FITC) - Anticorps de contrôle isotype
Mouse IgG (AF555) - Anticorps de contrôle isotype
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - Anticorps secondaire, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - Anticorps primaire, specific to FXYD5
Recombinant Vinculin Antibody - Anticorps primaire, specific to Vinculin
Mouse IgG2a-kappa (AF405), anti-A29L - Anticorps de contrôle isotype, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - Anticorps de contrôle isotype, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - Anticorps de contrôle isotype, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - Anticorps de contrôle isotype, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - Anticorps de contrôle isotype, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - Anticorps de contrôle isotype, specific to A29L
Mouse IgG3-kappa (Biotin), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - Anticorps secondaire
Goat Anti-Mouse IgG H&L (PE-Cy7) - Anticorps secondaire
Mouse IgG (PE-Cy5.5) - Anticorps de contrôle isotype
Goat Anti-Mouse IgG H&L (AF750) - Anticorps secondaire
Mouse IgG2a-kappa (AF555), anti-A29L - Anticorps de contrôle isotype, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - Anticorps de contrôle isotype, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - Anticorps secondaire
Goat Anti-Mouse IgG H&L (Oregon Green 488) - Anticorps secondaire
Goat Anti-Mouse IgG H&L (Cy3) - Anticorps secondaire
Mouse IgG2a-kappa (APC), anti-A29L - Anticorps de contrôle isotype, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - Anticorps de contrôle isotype, specific to HEL
Mouse IgG (PE-AF647) - Anticorps de contrôle isotype
Vinculin Mouse mAb (HRP) - Anticorps primaire, specific to Vinculin

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